You'll have to do this in batches of 5-10 cups since I'm assuming you got a tiny little SAB (haha I know) Now very carefully and slowly and steadily, start pouring the agar into the pp cups and keep closing/sealing the pp cups with the other hand and setting them aside. Spray everything again and again like you got bad OCD. Now iso spray the crap out of your media bottle, PP Cup stack, and your SAB, and prep your SAB for work. If you don't have a way to measure the temp on your bottle (if you don't have a laser thermometer) just set a timer for about 25-30 mins after taking it out of the PC and see if it's manageable to touch. I like to pour mine slightly hotter 45-50C. Ideally, you wanna pour the agar once it's cooled down to about 40-45C. Now once you take out the agar off your PC, it'll be crazy hot (obviously, so use mits), and then wait for it to start cooling. Sterilize your agar in the PC for at least 20 minutes (excluding 10 min venting time) You can make a trivet out of jar lids, or use a chapati/roti rack/stand, they work well. Once your media looks ready to be sterilized move on to the next step.Īlright so now take your media bottle and place it in your pressure cooker with a trivet and water (make sure you place the bottle on top of a trivet, never let the glass bottom of the bottle touch the bare base of the pressure cooker else your media bottle will burst) Pour 1-2 drops of coloring into the mix and swirl gently after screwing the cap halfway. ![]() Now, use a simple food-based coloring to add some color to your agar mixture. Now take it off the heat and pour it into a media bottle using a funnel. Immediately turn off the heat when it reaches a boil since you don't wanna overcook the agar. Now mix it all up together in a pot and bring the mix to a light bubbly boil. Measure out 10g agar and 7.5g malt extract for every 500 ml water. Wrap them in foil and pressure cook them for 15-20 mins. Take your PP cups or polypropylene empty Petris and stack em together. They aren't sterile right out of the factory and if you don't wanna lose half the cups you pour, don't skip this step. You will need to run a 15-20 min PC cycle on your PP cups before you pour agar on em. ![]() Polypropylene (PP) Microwavable food containers (cups) 50-100mL | Sterile petri dishesįeeling motivated enough to start your agar journey? Cool, let's begin.Pressure Cooker (Minimum 5L) (yo mama's regular old pressure cooker might just work fine as long as it reaches 15 psi or 1 kgf/cm 2 check the lid or weight).Proper storage and usage (usage you can search on YT - "Agar transfers in SAB").Making the agar properly with the correct ingredients.The only due diligence this requires is. Agar will take your mycology game to the next level, almost instantaneously. You can use agar for cloning live tissue cultures of mushrooms, you can use agar to spot contaminations in your cultures and syringes, you can use agar to clean up on contamination, to germinate spores, and to make infinite copies of your genetics. If you're even a TINY bit serious about mycology, agar can do wonders and beyond. I often see people struggling with agar and shying away from even trying it out because it seems super complicated and time-consuming, at least the way it's explained in most mainstream sources. ![]() The first tek is here friends! Mush love everyone ❤️
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